Automatic Mode Switching for A Multi-functional Variable Refrigerant Flow System

Multi-functional variable refrigerant flow system (MFVRF) is designed to realize simultaneous heating and cooling for individual zones. It is desirable to use existing measurements to determine switching between different modes under changes of ambient and load conditions, i.e. reversing the mode of indoor unit (IDU) and/or outdoor unit (ODU) heat exchangers (HX), as well as the bumpless transfer for controller switching. In this study, a set of mode switching logic is proposed for a four-zone MFVRF system, which involves both IDU and ODU mode switching actions. For the ODU-HX mode switching, thermodynamic analysis under different load changes reveals the qualitative trend for the air-side and refrigerant-side characteristics as the operation approaches to marginal scenarios. The ODU mode switching is thus based on the air-side temperature difference. Mode switching involving IDU action only is studied with a 1H3C (one heating three cooling) mode, in which IDU-1 is in heating mode and IDU-2, IDU-3 and IDU-4 are in cooling mode. For a given zone load conditions, when the zone temperature of IDU is higher than upper limit of a preset cooling mode hysteresis band, IDU enters the cooling mode by simultaneously opening all related cooling mode valves and closing heating-mode valves within time duration. On the other hand, the cooling mode is turned off by closing all related cooling valves when the zone temperature is lower than the lower limit of the cooling mode temperature band. Similarly, when the zone temperature is lower than lower limit of heating mode temperature band, IDU enters its heating mode. When the zone temperature for IDU is higher than the upper limit of heating mode temperature band, the heating mode is turned off. For ODU Mode Switching, it is proposed in this paper to use the temperature difference between the inlet and outlet air of the ODU HX. To justify the use of ODU air-side temperature differential as the indicator variable for ODU mode switching, several cases of 2H2C (two-heating two-cooling) mode are first simulated, in which the IDU-1 and IDU-2 are operated in heating mode and IDU-3 and IDU-4 are operated in cooling mode. A negative ramp of load change applied to IDU-3 within 1000 seconds. For the ODU-HX, the air inlet temperature is fixed at the ambient 20oC, while the air outlet temperature approaches closer and closer to 20oC under reducing cooling load in IDU-3. Simulation results have revealed the decreasing trend of COP. The T-s diagram for the refrigerant cycle of 2H2C mode is evaluated under several scenarios of reduction in IDU-3 cooling load. It reveals that a decreasing temperature difference at the air side or refrigerant side can be candidate probing variables for mode switching of ODU HX. Also, similar study is conducted when the ODU HX works as evaporator, with the MFVRF system operated in 3H1C (three heating one cooling) mode. Simulations for ODU HX mode switch case have been performed, and the results validate the effectiveness of the proposed scheme of mode switching

Model-free Control and Automatic Staging of Variable Refrigerant Flow System with Multiple Outdoor Units

For efficient operation of a variable refrigerant flow (VRF) air conditioning system with multiple outdoor units (ODUs), we propose a model-free control strategy based on extremum seeking control along with automatic staging control logic. The proposed strategy is evaluated with a representative VRF system consisting of 12 indoor units (IDUs) and three ODUs. The IDU zone temperature is regulated by EEV opening, and the compressor pressure is regulated by compressor speed. To optimize load sharing among multiple ODUs in operation, a set of bypass valves (BPVs) are added to the suction side of the compressors to manipulate refrigerant flow distribution among different compressors as needed. A penalty-function based multivariable extremum seeking control (ESC) method is used for real-time optimization of system operation. The performance index as the ESC feedback is the total power of the compressors, the ODU fans and the IDU fans, augmented with penalties for securing minimum superheat at the suction side of compressors. The manipulated inputs include the compressor suction pressure setpoint, the openings of BPVs at the suction side of the compressors, and a uniform setpoint of fan speed for all ODUs. As for the ESC feedback, the compressor power is normalized by its capacity. A set of control strategies for staging on/off particular ODUs is developed based on the compressor speed of the operating ODUs. Under increasing load, if the operating compressor(s) speed exceeds the higher limit of operation speed range (80% of rated speed), an additional ODU turned on to meet the load demand. Under decreasing load, it is desirable to turn off the least efficient ODU in a model-free fashion. In this study, an ESC based ODU staging-off strategy is proposed, for which the compressor shaft power normalized by the rated capacity is adopted as the ESC input. In addition to the compressor pressure setpoints and ODU fan speeds, the manipulated inputs of ESC also include the openings of suction-side BPVs in order to optimize load sharing among the multiple ODUs. With online optimization of ODU load sharing based on the normalized compressor power, the ESC can drive less efficient compressor(s) to operate at lower speed/capacity. If the compressor speed of an ODU falls below the preset lower limit of operational speed range (e.g. 20% of the rated speed) for long enough time, this ODU will be turned off. A dynamic simulation model of the multi-ODU VRF system is developed with Dymola and TIL Library. Simulation studies have been performed to evaluate the proposed ESC strategy for energy efficient operation during constant load patterns and the control logic for staging on and off ODU during load increase and decrease. The total power searched by the ESC is shown to be close to that obtained by a genetic algorithm based global optimization procedure in Dymola. Also, ESC is shown to be able to turn off least efficient ODU during load decrease without model knowledge. The load-sharing BPV at the compressor suction-side demonstrates bearable pressure loss except for the scenarios of large split ratio

Distributed Extremum Seeking Control for a Variable Refrigerant Flow System

The variable refrigerant flow (VRF) technology has facilitated the development of multi-split ductless air conditioning systems, in which multiple indoor units (IDU) are used to regulate the refrigerant flow to achieve individualized zoning control. Model based control for VRF system demands for more modeling efforts in part due to diverse configuration, as well as changes in load and ambient conditions. As a model-free control strategy, Extremum Seeking Control (ESC) has been investigated for VRF systems. Dong et al. (2015) applied the standard centralized ESC scheme to a VRF system that consists of one outdoor unit (ODU) and four IDU’s. Simulation results have indicated the effectiveness of such strategy. As the number of IDU’s increases, the complexity of centralized controllers will increase accordingly. Therefore distributed ESC becomes a natural consideration for VRF systems with large number of IDU’s. In this paper, the Shashahani gradient based distributed ESC scheme proposed by Poveda and Quijano (2013, 2015), is applied to the four-zone VRF system simulated by Dong et al. (2015). In particular, this scheme is enhanced by appending a band-pass filter array at the output to achieve a better “isolation†among individual input channels. A single-input ESC is applied to the ODU, while the distributed ESC is applied to the four IDU’s with each acting as an agent. For each agent, the respective power consumption is used as feedback. The objective is to minimize the total power consumption of all agents. For the ODU ESC, the compressor suction pressure (PCS) set-point is employed as the manipulative input. For the IDU DESC, the evaporator superheat (SH) set-point is used as the manipulative input for each IDU agent. The distributed ESC scheme assumes full information communication among all IDU’s. Simulation study is performed to evaluate the proposed strategy with the Modelica based dynamic simulation model developed by Dong et al. (2015). The ESC is designed under the ambient condition of 35oC and 40 %RH, respectively. The initial temperature of all four IDUs zone is 29oC, and the zone temperature set-point is 26oC. The heat loads for IDU1 through IDU4 are 3000W, 2600W, 2400W and 2000W, respectively. It takes the average total power about 10000 seconds to converge to about 3200W in steady state, with PCS around 13bar, and the SH values of IDU1 through IDU4 at 4.5oC, 4.5oC, 6oC, and 5.5oC, respectively. The total power consumption was decreased from 4500 W to 3200 W, i.e. by 29%. In comparison with the centralized ESC Dong et al. (2015), the steady state error of total power is less than 50w. Work is under way to improve transient and steady-state performance, as well as simulation of other operation modes.  Â

Molecular characterization and analysis of a novel protein disulfide isomerase-like protein of Eimeria tenella.

Protein disulfide isomerase (PDI) and PDI-like proteins are members of the thioredoxin superfamily. They contain thioredoxin-like domains and catalyze the physiological oxidation, reduction and isomerization of protein disulfide bonds, which are involved in cell function and development in prokaryotes and eukaryotes. In this study, EtPDIL, a novel PDI-like gene of Eimeria tenella, was cloned using rapid amplification of cDNA ends (RACE) according to the expressed sequence tag (EST). The EtPDIL cDNA contained 1129 nucleotides encoding 216 amino acids. The deduced EtPDIL protein belonged to thioredoxin-like superfamily and had a single predicted thioredoxin domain with a non-classical thioredoxin-like motif (SXXC). BLAST analysis showed that the EtPDIL protein was 55-59% identical to PDI-like proteins of other apicomplexan parasites. The transcript and protein levels of EtPDIL at different development stages were investigated by real-time quantitative PCR and western blot. The messenger RNA and protein levels of EtPDIL were higher in sporulated oocysts than in unsporulated oocysts, sporozoites or merozoites. Protein expression was barely detectable in unsporulated oocysts. Western blots showed that rabbit antiserum against recombinant EtPDIL recognized only a native 24 kDa protein from parasites. Immunolocalization with EtPDIL antibody showed that EtPDIL had a disperse distribution in the cytoplasm of whole sporozoites and merozoites. After sporozoites were incubated in complete medium, EtPDIL protein concentrated at the anterior of the sporozoites and appeared on the surface of parasites. Specific staining was more intense and mainly located on the parasite surface after merozoites released from mature schizonts invaded DF-1 cells. After development of parasites in DF-1 cells, staining intensified in trophozoites, immature schizonts and mature schizonts. Antibody inhibition of EtPDIL function reduced the ability of E. tenella to invade DF-1 cells. These results suggested that EtPDIL might be involved in sporulation in external environments and in host cell adhesion, invasion and development of E. tenella

Inhibition of sporozoite invasion <i>in vitro</i> by antibody against rEtPDIL.

<p>Anti- rEtPDIL, rabbit antiserum against recombinant EtPDIL protein; NA, naive rabbit serum. All assays were performed in triplicate. ** P<0.01 for differences between treatment with antibody against rEtPDIL and naïve rabbit serum at the same IgG concentration.</p

Western blots.

<p>(A) Purified recombinant EtPDIL (diaminobenzidine as substrate). Rabbit sera against sporulated oocysts of <i>E. tenella</i> or anti-GST monoclonal antibody was used as primary antibody. Lane 1, protein marker. Lane 2, anti-GST monoclonal. Lane 3, antisporulated-oocysts serum. Lane 4, naive rabbit serum. (B) Protein lysates from four different life cycle stages of <i>E. tenella.</i> Rabbit sera against rEtPDIL or mouse monoclonal anti-α-tubulin was used as primary antibody. Spz, sporozoites; SO, sporulated oocysts; UO, unsporulated oocysts; Mrz, merozoites.</p

Multiple alignment analysis of EtPDIL of <i>Eimera tenella</i> with PDIL from other apicomplexan parasites.

<p>Shown are sequences from <i>Plasmodium cynomolgi</i> (XP_004221713), <i>Plasmodium vivax</i> (XP_001614725), <i>Eimeria mitis</i> (CDJ34317.1). Deduced protein sequences were used in the Clustal W sequence alignment program. Asterisks, identical amino acids.</p

Quantitative real-time RT-PCR of EtPDIL expression in <i>E. tenella</i> developmental stages.

<p>UO, unsporulated oocysts; SO, sporulated oocysts; Spz, sporozoites; Mrz, merozoites. Bars not sharing the same letters were significantly different (P<0.05).</p

Full cDNA and deduced amino acid sequence of EtPDIL.

<p>Underlined, start and stop codons; double underlined, CAAT box in the 5′-UTR; gray, non-classical thioredoxin-like motif (SXXC); wavy underlined, N-myristoylation site.</p